Detailed information of TA system TA044264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4689863..4690465
• Replicon: chromosome
• Accession: NZ_AP027957
• Organism: Escherichia coli strain VE-T10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QQK14_RS22820
• Protein ID: WP_000897305.1
• Coordinates: 4690154..4690465 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QQK14_RS22815
• Protein ID: WP_000356397.1
• Coordinates: 4689863..4690153 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK14_RS22790 (4685808)	4685808..4686710	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
QQK14_RS22795 (4686707)	4686707..4687342	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QQK14_RS22800 (4687339)	4687339..4688268	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QQK14_RS22805 (4688598)	4688598..4688840	-	243	WP_001087409.1	protein YiiF	-
QQK14_RS22810 (4689059)	4689059..4689277	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QQK14_RS22815 (4689863)	4689863..4690153	-	291	WP_000356397.1	NadS family protein	Antitoxin
QQK14_RS22820 (4690154)	4690154..4690465	-	312	WP_000897305.1	hypothetical protein	Toxin
QQK14_RS22825 (4690694)	4690694..4691602	+	909	WP_001162704.1	alpha/beta hydrolase	-
QQK14_RS22830 (4691666)	4691666..4692607	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QQK14_RS22835 (4692652)	4692652..4693089	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QQK14_RS22840 (4693086)	4693086..4693958	-	873	WP_000920762.1	virulence factor BrkB family protein	-
QQK14_RS22845 (4693952)	4693952..4694551	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T44264 WP_000897305.1 NZ_AP027957:c4690465-4690154 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T44264 NZ_AP027957:c4690465-4690154 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT44264 WP_000356397.1 NZ_AP027957:c4690153-4689863 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT44264 NZ_AP027957:c4690153-4689863 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7