Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3824673..3825214 Replicon chromosome
Accession NZ_AP027957
Organism Escherichia coli strain VE-T10

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag QQK14_RS18730 Protein ID WP_000615982.1
Coordinates 3824936..3825214 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QQK14_RS18725 Protein ID WP_000729704.1
Coordinates 3824673..3824933 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK14_RS18700 (3819709) 3819709..3820848 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
QQK14_RS18705 (3820832) 3820832..3822925 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
QQK14_RS18710 (3823030) 3823030..3823308 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QQK14_RS18715 (3823301) 3823301..3823657 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
QQK14_RS18720 (3823714) 3823714..3824487 - 774 WP_000543899.1 C40 family peptidase -
QQK14_RS18725 (3824673) 3824673..3824933 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QQK14_RS18730 (3824936) 3824936..3825214 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QQK14_RS18735 (3825370) 3825370..3826110 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QQK14_RS18740 (3826081) 3826081..3826848 - 768 WP_000333380.1 class II glutamine amidotransferase -
QQK14_RS18745 (3826953) 3826953..3827531 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T44259 WP_000615982.1 NZ_AP027957:3824936-3825214 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T44259 NZ_AP027957:3824936-3825214 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT44259 WP_000729704.1 NZ_AP027957:3824673-3824933 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT44259 NZ_AP027957:3824673-3824933 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References