Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3728420..3728961 Replicon chromosome
Accession NZ_AP027953
Organism Escherichia coli strain VE-F5

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag QQJ99_RS18355 Protein ID WP_000615983.1
Coordinates 3728683..3728961 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag QQJ99_RS18350 Protein ID WP_000729703.1
Coordinates 3728420..3728680 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQJ99_RS18330 (3724095) 3724095..3724880 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QQJ99_RS18335 (3724852) 3724852..3726564 + 1713 Protein_3587 flagellar biosynthesis protein FlhA -
QQJ99_RS18340 (3726788) 3726788..3727285 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
QQJ99_RS18345 (3727461) 3727461..3728210 - 750 WP_000056849.1 C40 family peptidase -
QQJ99_RS18350 (3728420) 3728420..3728680 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QQJ99_RS18355 (3728683) 3728683..3728961 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QQJ99_RS18360 (3729117) 3729117..3729857 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QQJ99_RS18365 (3729828) 3729828..3730595 - 768 WP_000333380.1 class II glutamine amidotransferase -
QQJ99_RS18370 (3730801) 3730801..3731379 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T44233 WP_000615983.1 NZ_AP027953:3728683-3728961 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T44233 NZ_AP027953:3728683-3728961 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT44233 WP_000729703.1 NZ_AP027953:3728420-3728680 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT44233 NZ_AP027953:3728420-3728680 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References