Detailed information of TA system TA044217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1870114..1870616
• Replicon: chromosome
• Accession: NZ_AP027953
• Organism: Escherichia coli strain VE-F5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: QQJ99_RS09075
• Protein ID: WP_000767829.1
• Coordinates: 1870362..1870616 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: QQJ99_RS09070
• Protein ID: WP_001259255.1
• Coordinates: 1870114..1870365 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQJ99_RS09045 (1865292)	1865292..1866359	-	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
QQJ99_RS09050 (1866359)	1866359..1867429	-	1071	WP_000108939.1	histidinol-phosphate transaminase	-
QQJ99_RS09055 (1867426)	1867426..1868730	-	1305	WP_001614652.1	histidinol dehydrogenase	-
QQJ99_RS09060 (1868736)	1868736..1869635	-	900	WP_000131777.1	ATP phosphoribosyltransferase	-
QQJ99_RS09065 (1869781)	1869781..1869831	-	51	WP_001364200.1	his operon leader peptide	-
QQJ99_RS09070 (1870114)	1870114..1870365	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
QQJ99_RS09075 (1870362)	1870362..1870616	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
QQJ99_RS09080 (1870699)	1870699..1871523	+	825	WP_000754749.1	SDR family oxidoreductase	-
QQJ99_RS09085 (1871569)	1871569..1872498	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
QQJ99_RS09090 (1872713)	1872713..1872775	+	63	WP_010723108.1	membrane protein YoeI	-
QQJ99_RS09095 (1872765)	1872765..1874123	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
QQJ99_RS09100 (1874302)	1874302..1875360	+	1059	WP_000492321.1	thiosulfate utilization transporter TsuA/YeeE	-
QQJ99_RS09105 (1875374)	1875374..1875601	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T44217 WP_000767829.1 NZ_AP027953:1870362-1870616 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T44217 NZ_AP027953:1870362-1870616 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT44217 WP_001259255.1 NZ_AP027953:1870114-1870365 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT44217 NZ_AP027953:1870114-1870365 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0