Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 612524..613323 Replicon chromosome
Accession NZ_AP027953
Organism Escherichia coli strain VE-F5

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag QQJ99_RS02960 Protein ID WP_000347275.1
Coordinates 612524..612988 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QQJ99_RS02965 Protein ID WP_001307405.1
Coordinates 612988..613323 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQJ99_RS02930 (607525) 607525..607959 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
QQJ99_RS02935 (607977) 607977..608855 - 879 WP_039268471.1 PTS N-acetylgalactosamine transporter subunit IID -
QQJ99_RS02940 (608845) 608845..609624 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
QQJ99_RS02945 (609635) 609635..610108 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
QQJ99_RS02950 (610131) 610131..611411 - 1281 WP_039268470.1 tagatose-bisphosphate aldolase subunit KbaZ -
QQJ99_RS02955 (611660) 611660..612469 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
QQJ99_RS02960 (612524) 612524..612988 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QQJ99_RS02965 (612988) 612988..613323 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QQJ99_RS02970 (613472) 613472..615043 - 1572 WP_001273752.1 galactarate dehydratase -
QQJ99_RS02975 (615418) 615418..616752 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
QQJ99_RS02980 (616768) 616768..617538 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T44211 WP_000347275.1 NZ_AP027953:c612988-612524 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T44211 NZ_AP027953:c612988-612524 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT44211 WP_001307405.1 NZ_AP027953:c613323-612988 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT44211 NZ_AP027953:c613323-612988 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References