Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3720386..3720927 Replicon chromosome
Accession NZ_AP027946
Organism Escherichia coli strain VE-F10

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag QQK65_RS18025 Protein ID WP_000615983.1
Coordinates 3720649..3720927 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag QQK65_RS18020 Protein ID WP_000729703.1
Coordinates 3720386..3720646 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK65_RS18000 (3716061) 3716061..3716846 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QQK65_RS18005 (3716818) 3716818..3718530 + 1713 Protein_3520 flagellar biosynthesis protein FlhA -
QQK65_RS18010 (3718754) 3718754..3719251 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
QQK65_RS18015 (3719427) 3719427..3720176 - 750 WP_000056849.1 C40 family peptidase -
QQK65_RS18020 (3720386) 3720386..3720646 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QQK65_RS18025 (3720649) 3720649..3720927 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QQK65_RS18030 (3721083) 3721083..3721823 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QQK65_RS18035 (3721794) 3721794..3722561 - 768 WP_000333380.1 class II glutamine amidotransferase -
QQK65_RS18040 (3722767) 3722767..3723345 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / tssA / hcp1/tssD1 3714189..3742375 28186


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T44204 WP_000615983.1 NZ_AP027946:3720649-3720927 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T44204 NZ_AP027946:3720649-3720927 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT44204 WP_000729703.1 NZ_AP027946:3720386-3720646 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT44204 NZ_AP027946:3720386-3720646 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References