Detailed information of TA system TA044190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1084263..1084990
• Replicon: chromosome
• Accession: NZ_AP027946
• Organism: Escherichia coli strain VE-F10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: QQK65_RS05320
• Protein ID: WP_001521139.1
• Coordinates: 1084263..1084574 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QQK65_RS05325
• Protein ID: WP_000126294.1
• Coordinates: 1084571..1084990 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK65_RS05295 (1080198)	1080198..1081907	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
QQK65_RS05300 (1081917)	1081917..1082459	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
QQK65_RS05305 (1082459)	1082459..1083226	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
QQK65_RS05310 (1083223)	1083223..1083633	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QQK65_RS05315 (1083626)	1083626..1084096	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QQK65_RS05320 (1084263)	1084263..1084574	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
QQK65_RS05325 (1084571)	1084571..1084990	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QQK65_RS05330 (1085069)	1085069..1086493	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
QQK65_RS05335 (1086502)	1086502..1087959	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QQK65_RS05340 (1088219)	1088219..1089229	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
QQK65_RS05345 (1089378)	1089378..1089905	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T44190 WP_001521139.1 NZ_AP027946:1084263-1084574 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T44190 NZ_AP027946:1084263-1084574 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT44190 WP_000126294.1 NZ_AP027946:1084571-1084990 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT44190 NZ_AP027946:1084571-1084990 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9