44128

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4120406..4120903 Replicon chromosome
Accession NZ_AP027445
Organism Escherichia coli strain EC291 isolate EC291

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE55_RS22000 Protein ID WP_001173343.1
Coordinates 4120406..4120579 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE55_RS22005 Protein ID WP_000398619.1
Coordinates 4120607..4120903 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE55_RS21990 (4117071) 4117071..4118528 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
QUE55_RS21995 (4118765) 4118765..4120282 + 1518 WP_001295074.1 lysine--tRNA ligase -
QUE55_RS22000 (4120406) 4120406..4120579 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE55_RS22005 (4120607) 4120607..4120903 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE55_RS22010 (4121130) 4121130..4121402 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QUE55_RS22015 (4121414) 4121414..4121644 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QUE55_RS22020 (4121825) 4121825..4123456 + 1632 WP_001216477.1 sensor histidine kinase -
QUE55_RS22025 (4123453) 4123453..4124172 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4111547..4120903 9356


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T44128 WP_001173343.1 NZ_AP027445:c4120579-4120406 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T44128 NZ_AP027445:c4120579-4120406 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT44128 WP_000398619.1 NZ_AP027445:c4120903-4120607 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT44128 NZ_AP027445:c4120903-4120607 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References