Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3606313..3606854 Replicon chromosome
Accession NZ_AP027445
Organism Escherichia coli strain EC291 isolate EC291

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag QUE55_RS19510 Protein ID WP_000615976.1
Coordinates 3606576..3606854 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QUE55_RS19505 Protein ID WP_000729704.1
Coordinates 3606313..3606573 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE55_RS19485 (3601979) 3601979..3603680 + 1702 Protein_3505 flagellar biosynthesis protein FlhA -
QUE55_RS19490 (3603904) 3603904..3604401 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
QUE55_RS19495 (3604577) 3604577..3605350 - 774 WP_015675129.1 C40 family peptidase -
QUE55_RS19500 (3605416) 3605416..3606113 + 698 WP_223367350.1 IS1 family transposase -
QUE55_RS19505 (3606313) 3606313..3606573 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QUE55_RS19510 (3606576) 3606576..3606854 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QUE55_RS19515 (3607010) 3607010..3607750 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QUE55_RS19520 (3607721) 3607721..3608488 - 768 WP_000333379.1 class II glutamine amidotransferase -
QUE55_RS19525 (3608694) 3608694..3609272 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3598388..3618125 19737
- flank IS/Tn - - 3605820..3606113 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T44125 WP_000615976.1 NZ_AP027445:3606576-3606854 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T44125 NZ_AP027445:3606576-3606854 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT44125 WP_000729704.1 NZ_AP027445:3606313-3606573 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT44125 NZ_AP027445:3606313-3606573 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References