Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3599350..3600044 | Replicon | chromosome |
| Accession | NZ_AP027445 | ||
| Organism | Escherichia coli strain EC291 isolate EC291 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | QUE55_RS19465 | Protein ID | WP_001263493.1 |
| Coordinates | 3599350..3599748 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | QUE55_RS19470 | Protein ID | WP_000554757.1 |
| Coordinates | 3599751..3600044 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3595010) | 3595010..3595090 | - | 81 | NuclAT_9 | - | - |
| - (3595010) | 3595010..3595090 | - | 81 | NuclAT_9 | - | - |
| - (3595010) | 3595010..3595090 | - | 81 | NuclAT_9 | - | - |
| - (3595010) | 3595010..3595090 | - | 81 | NuclAT_9 | - | - |
| QUE55_RS19435 (3594350) | 3594350..3595594 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| QUE55_RS19440 (3595686) | 3595686..3596144 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| QUE55_RS19445 (3596405) | 3596405..3597862 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| QUE55_RS19450 (3597919) | 3597919..3598440 | - | 522 | Protein_3498 | peptide chain release factor H | - |
| QUE55_RS19455 (3598439) | 3598439..3598642 | - | 204 | Protein_3499 | RtcB family protein | - |
| QUE55_RS19460 (3598888) | 3598888..3599340 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| QUE55_RS19465 (3599350) | 3599350..3599748 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| QUE55_RS19470 (3599751) | 3599751..3600044 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| QUE55_RS19475 (3600096) | 3600096..3601151 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| QUE55_RS19480 (3601222) | 3601222..3602007 | - | 786 | WP_000207587.1 | putative lateral flagellar export/assembly protein LafU | - |
| QUE55_RS19485 (3601979) | 3601979..3603680 | + | 1702 | Protein_3505 | flagellar biosynthesis protein FlhA | - |
| QUE55_RS19490 (3603904) | 3603904..3604401 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | gmhA/lpcA | 3598388..3618125 | 19737 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T44124 WP_001263493.1 NZ_AP027445:c3599748-3599350 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T44124 NZ_AP027445:c3599748-3599350 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT44124 WP_000554757.1 NZ_AP027445:c3600044-3599751 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT44124 NZ_AP027445:c3600044-3599751 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|