Detailed information of TA system TA044097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4342186..4342913
• Replicon: chromosome
• Accession: NZ_AP027440
• Organism: Escherichia coli strain EC556 isolate EC556

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: QUE77_RS22730
• Protein ID: WP_000547555.1
• Coordinates: 4342186..4342497 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE77_RS22735
• Protein ID: WP_000126294.1
• Coordinates: 4342494..4342913 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE77_RS22705 (4338121)	4338121..4339830	+	1710	WP_286338270.1	formate hydrogenlyase subunit HycE	-
QUE77_RS22710 (4339840)	4339840..4340382	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
QUE77_RS22715 (4340382)	4340382..4341149	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
QUE77_RS22720 (4341146)	4341146..4341556	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
QUE77_RS22725 (4341549)	4341549..4342019	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QUE77_RS22730 (4342186)	4342186..4342497	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
QUE77_RS22735 (4342494)	4342494..4342913	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QUE77_RS22740 (4342992)	4342992..4344416	-	1425	WP_063086210.1	6-phospho-beta-glucosidase AscB	-
QUE77_RS22745 (4344425)	4344425..4345882	-	1458	WP_001107888.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QUE77_RS22750 (4346142)	4346142..4347152	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
QUE77_RS22755 (4347301)	4347301..4347828	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T44097 WP_000547555.1 NZ_AP027440:4342186-4342497 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T44097 NZ_AP027440:4342186-4342497 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT44097 WP_000126294.1 NZ_AP027440:4342494-4342913 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT44097 NZ_AP027440:4342494-4342913 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGATACTAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAATAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2