Detailed information of TA system TA044093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2984282..2984884
• Replicon: chromosome
• Accession: NZ_AP027440
• Organism: Escherichia coli strain EC556 isolate EC556

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUE77_RS16210
• Protein ID: WP_000897305.1
• Coordinates: 2984573..2984884 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE77_RS16205
• Protein ID: WP_000356395.1
• Coordinates: 2984282..2984572 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE77_RS16170 (2979905)	2979905..2980807	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
QUE77_RS16175 (2980804)	2980804..2981439	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUE77_RS16180 (2981436)	2981436..2982365	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QUE77_RS16185 (2982547)	2982547..2982789	-	243	WP_001306649.1	protein YiiF	-
QUE77_RS16190 (2983008)	2983008..2983226	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QUE77_RS16195 (2983645)	2983645..2983923	-	279	WP_001306650.1	hypothetical protein	-
QUE77_RS16200 (2983985)	2983985..2984197	-	213	WP_000197769.1	hypothetical protein	-
QUE77_RS16205 (2984282)	2984282..2984572	-	291	WP_000356395.1	NadS family protein	Antitoxin
QUE77_RS16210 (2984573)	2984573..2984884	-	312	WP_000897305.1	hypothetical protein	Toxin
QUE77_RS16215 (2985113)	2985113..2986021	+	909	WP_001306651.1	alpha/beta hydrolase	-
QUE77_RS16220 (2986190)	2986190..2987104	-	915	WP_225102955.1	transposase	-
QUE77_RS16225 (2987117)	2987117..2988004	-	888	Protein_2912	hypothetical protein	-
QUE77_RS16230 (2988420)	2988420..2989361	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUE77_RS16235 (2989406)	2989406..2989843	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T44093 WP_000897305.1 NZ_AP027440:c2984884-2984573 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T44093 NZ_AP027440:c2984884-2984573 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT44093 WP_000356395.1 NZ_AP027440:c2984572-2984282 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT44093 NZ_AP027440:c2984572-2984282 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7