Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2167441..2168135 Replicon chromosome
Accession NZ_AP027440
Organism Escherichia coli strain EC556 isolate EC556

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag QUE77_RS12305 Protein ID WP_001521903.1
Coordinates 2167441..2167839 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag QUE77_RS12310 Protein ID WP_000554755.1
Coordinates 2167842..2168135 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE77_RS12275 (2162575) 2162575..2164032 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
QUE77_RS12280 (2164041) 2164041..2164322 + 282 WP_077881290.1 hypothetical protein -
QUE77_RS12285 (2164339) 2164339..2164848 - 510 WP_063085380.1 metal-dependent hydrolase -
QUE77_RS12290 (2164910) 2164910..2165524 - 615 WP_000602129.1 peptide chain release factor H -
QUE77_RS12295 (2165521) 2165521..2166660 - 1140 WP_191667308.1 RNA ligase RtcB family protein -
QUE77_RS12300 (2166979) 2166979..2167431 - 453 WP_001059858.1 GNAT family N-acetyltransferase -
QUE77_RS12305 (2167441) 2167441..2167839 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QUE77_RS12310 (2167842) 2167842..2168135 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QUE77_RS12315 (2168187) 2168187..2169242 - 1056 WP_001226166.1 DNA polymerase IV -
QUE77_RS12320 (2169313) 2169313..2170098 - 786 WP_074150236.1 putative lateral flagellar export/assembly protein LafU -
QUE77_RS12325 (2170070) 2170070..2171782 + 1713 Protein_2159 flagellar biosynthesis protein FlhA -
QUE77_RS12330 (2171887) 2171887..2172165 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QUE77_RS12335 (2172158) 2172158..2172514 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T44089 WP_001521903.1 NZ_AP027440:c2167839-2167441 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T44089 NZ_AP027440:c2167839-2167441 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT44089 WP_000554755.1 NZ_AP027440:c2168135-2167842 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT44089 NZ_AP027440:c2168135-2167842 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4


Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References