Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 104967..105469 Replicon chromosome
Accession NZ_AP027440
Organism Escherichia coli strain EC556 isolate EC556

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag QUE77_RS01840 Protein ID WP_000767829.1
Coordinates 105215..105469 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag QUE77_RS01835 Protein ID WP_001259255.1
Coordinates 104967..105218 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE77_RS01810 (100145) 100145..101212 - 1068 WP_286338295.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
QUE77_RS01815 (101212) 101212..102282 - 1071 WP_063085464.1 histidinol-phosphate transaminase -
QUE77_RS01820 (102279) 102279..103583 - 1305 WP_063085463.1 histidinol dehydrogenase -
QUE77_RS01825 (103589) 103589..104488 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
QUE77_RS01830 (104634) 104634..104684 - 51 WP_001364200.1 his operon leader peptide -
QUE77_RS01835 (104967) 104967..105218 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
QUE77_RS01840 (105215) 105215..105469 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
QUE77_RS01845 (105552) 105552..106376 + 825 WP_000754737.1 SDR family oxidoreductase -
QUE77_RS01850 (106422) 106422..107351 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
QUE77_RS01855 (107566) 107566..107628 + 63 WP_010723108.1 membrane protein YoeI -
QUE77_RS01860 (107618) 107618..108976 + 1359 WP_248787450.1 putrescine/proton symporter PlaP -
QUE77_RS01865 (109190) 109190..109648 + 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 109190..109648 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T44073 WP_000767829.1 NZ_AP027440:105215-105469 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T44073 NZ_AP027440:105215-105469 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT44073 WP_001259255.1 NZ_AP027440:104967-105218 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT44073 NZ_AP027440:104967-105218 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References