Detailed information of TA system TA044067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4340468..4341195
• Replicon: chromosome
• Accession: NZ_AP027436
• Organism: Escherichia coli strain EC571 isolate EC571

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: QUE57_RS22705
• Protein ID: WP_000547555.1
• Coordinates: 4340468..4340779 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE57_RS22710
• Protein ID: WP_000126294.1
• Coordinates: 4340776..4341195 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE57_RS22680 (4336403)	4336403..4338112	+	1710	WP_286338270.1	formate hydrogenlyase subunit HycE	-
QUE57_RS22685 (4338122)	4338122..4338664	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
QUE57_RS22690 (4338664)	4338664..4339431	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
QUE57_RS22695 (4339428)	4339428..4339838	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
QUE57_RS22700 (4339831)	4339831..4340301	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QUE57_RS22705 (4340468)	4340468..4340779	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
QUE57_RS22710 (4340776)	4340776..4341195	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QUE57_RS22715 (4341274)	4341274..4342698	-	1425	WP_063086210.1	6-phospho-beta-glucosidase AscB	-
QUE57_RS22720 (4342707)	4342707..4344164	-	1458	WP_001107888.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QUE57_RS22725 (4344424)	4344424..4345434	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
QUE57_RS22730 (4345583)	4345583..4346110	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T44067 WP_000547555.1 NZ_AP027436:4340468-4340779 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T44067 NZ_AP027436:4340468-4340779 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT44067 WP_000126294.1 NZ_AP027436:4340776-4341195 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT44067 NZ_AP027436:4340776-4341195 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGATACTAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAATAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2