Detailed information of TA system TA044063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2982564..2983166
• Replicon: chromosome
• Accession: NZ_AP027436
• Organism: Escherichia coli strain EC571 isolate EC571

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUE57_RS16185
• Protein ID: WP_000897305.1
• Coordinates: 2982855..2983166 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE57_RS16180
• Protein ID: WP_000356395.1
• Coordinates: 2982564..2982854 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE57_RS16145 (2978187)	2978187..2979089	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
QUE57_RS16150 (2979086)	2979086..2979721	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUE57_RS16155 (2979718)	2979718..2980647	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QUE57_RS16160 (2980829)	2980829..2981071	-	243	WP_001306649.1	protein YiiF	-
QUE57_RS16165 (2981290)	2981290..2981508	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QUE57_RS16170 (2981927)	2981927..2982205	-	279	WP_001306650.1	hypothetical protein	-
QUE57_RS16175 (2982267)	2982267..2982479	-	213	WP_000197769.1	hypothetical protein	-
QUE57_RS16180 (2982564)	2982564..2982854	-	291	WP_000356395.1	NadS family protein	Antitoxin
QUE57_RS16185 (2982855)	2982855..2983166	-	312	WP_000897305.1	hypothetical protein	Toxin
QUE57_RS16190 (2983395)	2983395..2984303	+	909	WP_001306651.1	alpha/beta hydrolase	-
QUE57_RS16195 (2984472)	2984472..2985386	-	915	WP_225102955.1	transposase	-
QUE57_RS16200 (2985399)	2985399..2986286	-	888	Protein_2910	hypothetical protein	-
QUE57_RS16205 (2986702)	2986702..2987643	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUE57_RS16210 (2987688)	2987688..2988125	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T44063 WP_000897305.1 NZ_AP027436:c2983166-2982855 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T44063 NZ_AP027436:c2983166-2982855 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT44063 WP_000356395.1 NZ_AP027436:c2982854-2982564 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT44063 NZ_AP027436:c2982854-2982564 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7