Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 38211..38475 Replicon plasmid pEC571_1
Accession NZ_AP027435
Organism Escherichia coli strain EC571 isolate EC571

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag QUE57_RS01080 Protein ID WP_001331364.1
Coordinates 38323..38475 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 38211..38273 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE57_RS01065 (33450) 33450..35741 - 2292 WP_137653291.1 F-type conjugative transfer protein TrbC -
QUE57_RS01070 (35734) 35734..36804 - 1071 WP_137653290.1 IncI1-type conjugal transfer protein TrbB -
QUE57_RS01075 (36823) 36823..38031 - 1209 WP_024171190.1 IncI1-type conjugal transfer protein TrbA -
- (38211) 38211..38273 - 63 NuclAT_0 - Antitoxin
- (38211) 38211..38273 - 63 NuclAT_0 - Antitoxin
- (38211) 38211..38273 - 63 NuclAT_0 - Antitoxin
- (38211) 38211..38273 - 63 NuclAT_0 - Antitoxin
QUE57_RS01080 (38323) 38323..38475 + 153 WP_001331364.1 Hok/Gef family protein Toxin
QUE57_RS01085 (38547) 38547..38798 - 252 WP_001291964.1 hypothetical protein -
QUE57_RS01090 (39297) 39297..39392 + 96 WP_281506735.1 DinQ-like type I toxin DqlB -
QUE57_RS01095 (39457) 39457..39633 - 177 WP_001054897.1 hypothetical protein -
QUE57_RS01100 (40025) 40025..40234 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
QUE57_RS01105 (40306) 40306..40956 - 651 WP_001178506.1 plasmid IncI1-type surface exclusion protein ExcA -
QUE57_RS01110 (41030) 41030..43198 - 2169 WP_206975440.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..84551 84551


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T44039 WP_001331364.1 NZ_AP027435:38323-38475 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T44039 NZ_AP027435:38323-38475 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT44039 NZ_AP027435:c38273-38211 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References