Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4260295..4260989 Replicon chromosome
Accession NZ_AP027432
Organism Escherichia coli strain EC581 isolate EC581

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag QUE97_RS23335 Protein ID WP_001521903.1
Coordinates 4260591..4260989 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag QUE97_RS23330 Protein ID WP_000554755.1
Coordinates 4260295..4260588 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE97_RS23305 (4255916) 4255916..4256272 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
QUE97_RS23310 (4256265) 4256265..4256543 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QUE97_RS23315 (4256648) 4256648..4258360 - 1713 Protein_4077 flagellar biosynthesis protein FlhA -
QUE97_RS23320 (4258332) 4258332..4259117 + 786 WP_074150236.1 putative lateral flagellar export/assembly protein LafU -
QUE97_RS23325 (4259188) 4259188..4260243 + 1056 WP_001226166.1 DNA polymerase IV -
QUE97_RS23330 (4260295) 4260295..4260588 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QUE97_RS23335 (4260591) 4260591..4260989 + 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QUE97_RS23340 (4260999) 4260999..4261451 + 453 WP_001059858.1 GNAT family N-acetyltransferase -
QUE97_RS23345 (4261770) 4261770..4262909 + 1140 WP_191667308.1 RNA ligase RtcB family protein -
QUE97_RS23350 (4262906) 4262906..4263520 + 615 WP_000602129.1 peptide chain release factor H -
QUE97_RS23355 (4263582) 4263582..4264091 + 510 WP_063085380.1 metal-dependent hydrolase -
QUE97_RS23360 (4264108) 4264108..4264389 - 282 WP_077881290.1 hypothetical protein -
QUE97_RS23365 (4264398) 4264398..4265855 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T44033 WP_001521903.1 NZ_AP027432:4260591-4260989 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T44033 NZ_AP027432:4260591-4260989 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT44033 WP_000554755.1 NZ_AP027432:4260295-4260588 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT44033 NZ_AP027432:4260295-4260588 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4


Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References