Detailed information of TA system TA044025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2085517..2086244
• Replicon: chromosome
• Accession: NZ_AP027432
• Organism: Escherichia coli strain EC581 isolate EC581

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: QUE97_RS12905
• Protein ID: WP_000547555.1
• Coordinates: 2085933..2086244 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE97_RS12900
• Protein ID: WP_000126294.1
• Coordinates: 2085517..2085936 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE97_RS12880 (2080602)	2080602..2081129	-	528	WP_001078777.1	electron transport protein HydN	-
QUE97_RS12885 (2081278)	2081278..2082288	-	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
QUE97_RS12890 (2082548)	2082548..2084005	+	1458	WP_001107888.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QUE97_RS12895 (2084014)	2084014..2085438	+	1425	WP_063086210.1	6-phospho-beta-glucosidase AscB	-
QUE97_RS12900 (2085517)	2085517..2085936	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QUE97_RS12905 (2085933)	2085933..2086244	-	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
QUE97_RS12910 (2086411)	2086411..2086881	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QUE97_RS12915 (2086874)	2086874..2087284	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
QUE97_RS12920 (2087281)	2087281..2088048	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
QUE97_RS12925 (2088048)	2088048..2088590	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
QUE97_RS12930 (2088600)	2088600..2090309	-	1710	WP_286338270.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T44025 WP_000547555.1 NZ_AP027432:c2086244-2085933 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T44025 NZ_AP027432:c2086244-2085933 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT44025 WP_000126294.1 NZ_AP027432:c2085936-2085517 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT44025 NZ_AP027432:c2085936-2085517 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGATACTAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAATAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2