Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1275126..1275628 Replicon chromosome
Accession NZ_AP027432
Organism Escherichia coli strain EC581 isolate EC581

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag QUE97_RS09010 Protein ID WP_000767829.1
Coordinates 1275126..1275380 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag QUE97_RS09015 Protein ID WP_001259255.1
Coordinates 1275377..1275628 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE97_RS08985 (1270947) 1270947..1271405 - 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -
QUE97_RS08990 (1271619) 1271619..1272977 - 1359 WP_248787450.1 putrescine/proton symporter PlaP -
QUE97_RS08995 (1272967) 1272967..1273029 - 63 WP_010723108.1 membrane protein YoeI -
QUE97_RS09000 (1273244) 1273244..1274173 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
QUE97_RS09005 (1274219) 1274219..1275043 - 825 WP_000754737.1 SDR family oxidoreductase -
QUE97_RS09010 (1275126) 1275126..1275380 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
QUE97_RS09015 (1275377) 1275377..1275628 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
QUE97_RS09020 (1275911) 1275911..1275961 + 51 WP_001364200.1 his operon leader peptide -
QUE97_RS09025 (1276107) 1276107..1277006 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
QUE97_RS09030 (1277012) 1277012..1278316 + 1305 WP_063085463.1 histidinol dehydrogenase -
QUE97_RS09035 (1278313) 1278313..1279383 + 1071 WP_063085464.1 histidinol-phosphate transaminase -
QUE97_RS09040 (1279383) 1279383..1280450 + 1068 WP_286338295.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1270947..1271405 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T44024 WP_000767829.1 NZ_AP027432:c1275380-1275126 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T44024 NZ_AP027432:c1275380-1275126 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT44024 WP_001259255.1 NZ_AP027432:c1275628-1275377 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT44024 NZ_AP027432:c1275628-1275377 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References