Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 38224..38488 Replicon plasmid pEC581_2
Accession NZ_AP027430
Organism Escherichia coli strain EC581 isolate EC581

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag QUE97_RS01080 Protein ID WP_001331364.1
Coordinates 38336..38488 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 38224..38286 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE97_RS01065 (33463) 33463..35754 - 2292 WP_137653291.1 F-type conjugative transfer protein TrbC -
QUE97_RS01070 (35747) 35747..36817 - 1071 WP_137653290.1 IncI1-type conjugal transfer protein TrbB -
QUE97_RS01075 (36836) 36836..38044 - 1209 WP_024171190.1 IncI1-type conjugal transfer protein TrbA -
- (38224) 38224..38286 - 63 NuclAT_0 - Antitoxin
- (38224) 38224..38286 - 63 NuclAT_0 - Antitoxin
- (38224) 38224..38286 - 63 NuclAT_0 - Antitoxin
- (38224) 38224..38286 - 63 NuclAT_0 - Antitoxin
QUE97_RS01080 (38336) 38336..38488 + 153 WP_001331364.1 Hok/Gef family protein Toxin
QUE97_RS01085 (38560) 38560..38811 - 252 WP_001291964.1 hypothetical protein -
QUE97_RS01090 (39310) 39310..39405 + 96 WP_281506735.1 DinQ-like type I toxin DqlB -
QUE97_RS01095 (39470) 39470..39646 - 177 WP_001054897.1 hypothetical protein -
QUE97_RS01100 (40038) 40038..40247 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
QUE97_RS01105 (40319) 40319..40969 - 651 WP_001178506.1 plasmid IncI1-type surface exclusion protein ExcA -
QUE97_RS01110 (41043) 41043..43211 - 2169 WP_206975440.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..83702 83702


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T44008 WP_001331364.1 NZ_AP027430:38336-38488 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T44008 NZ_AP027430:38336-38488 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT44008 NZ_AP027430:c38286-38224 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References