43993

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4235076..4235573 Replicon chromosome
Accession NZ_AP027424
Organism Escherichia coli strain B27 isolate B27

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE78_RS20915 Protein ID WP_001173343.1
Coordinates 4235076..4235249 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE78_RS20920 Protein ID WP_000398619.1
Coordinates 4235277..4235573 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE78_RS20905 (4231746) 4231746..4233203 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
QUE78_RS20910 (4233440) 4233440..4234957 + 1518 WP_001295074.1 lysine--tRNA ligase -
QUE78_RS20915 (4235076) 4235076..4235249 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE78_RS20920 (4235277) 4235277..4235573 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE78_RS20925 (4235800) 4235800..4236072 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QUE78_RS20930 (4236084) 4236084..4236314 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QUE78_RS20935 (4236495) 4236495..4238126 + 1632 WP_001216477.1 sensor histidine kinase -
QUE78_RS20940 (4238123) 4238123..4238842 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4226222..4235573 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43993 WP_001173343.1 NZ_AP027424:c4235249-4235076 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43993 NZ_AP027424:c4235249-4235076 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43993 WP_000398619.1 NZ_AP027424:c4235573-4235277 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43993 NZ_AP027424:c4235573-4235277 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References