Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 705766..706493 Replicon chromosome
Accession NZ_AP027424
Organism Escherichia coli strain B27 isolate B27

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag QUE78_RS03455 Protein ID WP_000550189.1
Coordinates 705766..706080 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QUE78_RS03460 Protein ID WP_149543356.1
Coordinates 706077..706493 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE78_RS03435 (701924) 701924..702910 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
QUE78_RS03440 (702989) 702989..703681 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
QUE78_RS03445 (703758) 703758..704261 - 504 WP_001300832.1 M48 family metallopeptidase -
QUE78_RS03450 (704346) 704346..705482 + 1137 WP_149543355.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
QUE78_RS03455 (705766) 705766..706080 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
QUE78_RS03460 (706077) 706077..706493 + 417 WP_149543356.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
QUE78_RS03465 (706538) 706538..708556 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
QUE78_RS03470 (708982) 708982..711333 - 2352 WP_000695489.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T43975 WP_000550189.1 NZ_AP027424:705766-706080 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T43975 NZ_AP027424:705766-706080 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT43975 WP_149543356.1 NZ_AP027424:706077-706493 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKITLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT43975 NZ_AP027424:706077-706493 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAAATAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References