Detailed information of TA system TA043973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4683107..4683709
• Replicon: chromosome
• Accession: NZ_AP027423
• Organism: Escherichia coli strain EC261 isolate EC261

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUE58_RS25290
• Protein ID: WP_000897305.1
• Coordinates: 4683107..4683418 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE58_RS25295
• Protein ID: WP_000356397.1
• Coordinates: 4683419..4683709 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE58_RS25265 (4679021)	4679021..4679620	+	600	WP_001295269.1	glucose-1-phosphatase	-
QUE58_RS25270 (4679614)	4679614..4680486	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QUE58_RS25275 (4680483)	4680483..4680920	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QUE58_RS25280 (4680965)	4680965..4681906	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUE58_RS25285 (4681970)	4681970..4682878	-	909	WP_001162704.1	alpha/beta hydrolase	-
QUE58_RS25290 (4683107)	4683107..4683418	+	312	WP_000897305.1	hypothetical protein	Toxin
QUE58_RS25295 (4683419)	4683419..4683709	+	291	WP_000356397.1	NadS family protein	Antitoxin
QUE58_RS25300 (4684295)	4684295..4684513	+	219	WP_001295676.1	CopG family transcriptional regulator	-
QUE58_RS25305 (4684732)	4684732..4684974	+	243	WP_001087409.1	protein YiiF	-
QUE58_RS25310 (4685304)	4685304..4686233	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QUE58_RS25315 (4686230)	4686230..4686865	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUE58_RS25320 (4686862)	4686862..4687764	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43973 WP_000897305.1 NZ_AP027423:4683107-4683418 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43973 NZ_AP027423:4683107-4683418 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43973 WP_000356397.1 NZ_AP027423:4683419-4683709 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43973 NZ_AP027423:4683419-4683709 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7