Detailed information of TA system TA043951

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2013135..2013506
• Replicon: chromosome
• Accession: NZ_AP027423
• Organism: Escherichia coli strain EC261 isolate EC261

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: QUE58_RS12225
• Protein ID: WP_001317028.1
• Coordinates: 2013312..2013506 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2013135..2013313 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE58_RS12195 (2008887)	2008887..2009060	+	174	WP_001296046.1	protein YnaL	-
QUE58_RS12200 (2009090)	2009090..2010463	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
QUE58_RS12205 (2010592)	2010592..2011527	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
QUE58_RS12210 (2011579)	2011579..2012814	-	1236	WP_000040852.1	site-specific integrase	-
QUE58_RS12215 (2012816)	2012816..2013031	-	216	WP_000079604.1	excisionase XisR	-
- (2013135)	2013135..2013313	+	179	NuclAT_0	-	Antitoxin
- (2013135)	2013135..2013313	+	179	NuclAT_0	-	Antitoxin
- (2013135)	2013135..2013313	+	179	NuclAT_0	-	Antitoxin
- (2013135)	2013135..2013313	+	179	NuclAT_0	-	Antitoxin
QUE58_RS12220 (2013110)	2013110..2013319	-	210	WP_021554369.1	double-strand break reduction protein RcbA	-
QUE58_RS12225 (2013312)	2013312..2013506	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
QUE58_RS12230 (2013563)	2013563..2014372	-	810	WP_000166319.1	recombination protein RecT	-
QUE58_RS12235 (2014365)	2014365..2016965	-	2601	WP_286347923.1	exodeoxyribonuclease VIII	-
QUE58_RS12240 (2017067)	2017067..2017342	-	276	WP_000632297.1	protein RacC	-
QUE58_RS12245 (2017417)	2017417..2017587	-	171	WP_001352098.1	YdaE family protein	-
QUE58_RS12250 (2017587)	2017587..2017808	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2011579..2039873	28294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T43951 WP_001317028.1 NZ_AP027423:c2013506-2013312 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T43951 NZ_AP027423:c2013506-2013312 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT43951 NZ_AP027423:2013135-2013313 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGTATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin