Detailed information of TA system TA043933

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 113714..114135
• Replicon: plasmid pEC261_2
• Accession: NZ_AP027421
• Organism: Escherichia coli strain EC261 isolate EC261

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QUE58_RS01080
• Protein ID: WP_096937776.1
• Coordinates: 113714..113839 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	113937..114135 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE58_RS01060 (108891)	108891..109751	-	861	WP_000704513.1	alpha/beta hydrolase	-
QUE58_RS01065 (109810)	109810..110556	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
QUE58_RS01070 (110576)	110576..112966	-	2391	Protein_107	conjugative transfer relaxase/helicase TraI	-
QUE58_RS01075 (113117)	113117..113413	+	297	Protein_108	hypothetical protein	-
QUE58_RS01080 (113714)	113714..113839	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
QUE58_RS01085 (113781)	113781..113930	-	150	Protein_110	DUF5431 family protein	-
- (113937)	113937..114135	-	199	NuclAT_0	-	Antitoxin
- (113937)	113937..114135	-	199	NuclAT_0	-	Antitoxin
- (113937)	113937..114135	-	199	NuclAT_0	-	Antitoxin
- (113937)	113937..114135	-	199	NuclAT_0	-	Antitoxin
QUE58_RS01090 (114104)	114104..114866	-	763	Protein_111	plasmid SOS inhibition protein A	-
QUE58_RS01095 (114863)	114863..115297	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
QUE58_RS01100 (115352)	115352..117310	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
QUE58_RS01105 (117376)	117376..117609	-	234	WP_000005990.1	DUF905 family protein	-
QUE58_RS01110 (117666)	117666..118205	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
QUE58_RS01115 (118523)	118523..119032	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sitABCD / tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..138688	138688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T43933 WP_096937776.1 NZ_AP027421:c113839-113714 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T43933 NZ_AP027421:c113839-113714 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT43933 NZ_AP027421:c114135-113937 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin