Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 2513..2767 Replicon plasmid pEC261_3
Accession NZ_AP027420
Organism Escherichia coli strain EC261 isolate EC261

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag QUE58_RS00030 Protein ID WP_001312851.1
Coordinates 2618..2767 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 2513..2574 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE58_RS00010 (67) 67..927 + 861 WP_000704513.1 alpha/beta hydrolase -
QUE58_RS00015 (1030) 1030..1590 + 561 WP_000139315.1 fertility inhibition protein FinO -
QUE58_RS00020 (1726) 1726..1938 + 213 WP_001299730.1 ANR family transcriptional regulator -
QUE58_RS00025 (2183) 2183..2257 + 75 Protein_4 endonuclease -
- (2513) 2513..2574 - 62 NuclAT_1 - Antitoxin
- (2513) 2513..2574 - 62 NuclAT_1 - Antitoxin
- (2513) 2513..2574 - 62 NuclAT_1 - Antitoxin
- (2513) 2513..2574 - 62 NuclAT_1 - Antitoxin
QUE58_RS00030 (2618) 2618..2767 + 150 WP_001312851.1 Hok/Gef family protein Toxin
QUE58_RS00035 (3051) 3051..3308 + 258 WP_000083845.1 replication regulatory protein RepA -
QUE58_RS00040 (3325) 3325..3576 - 252 WP_223195197.1 replication protein RepA -
QUE58_RS00045 (3567) 3567..3614 + 48 WP_229471593.1 hypothetical protein -
QUE58_RS00050 (3607) 3607..4089 + 483 WP_001273588.1 hypothetical protein -
QUE58_RS00055 (4082) 4082..4547 + 466 Protein_10 plasmid replication initiator RepA -
QUE58_RS00060 (4480) 4480..7485 - 3006 WP_001143760.1 Tn3-like element Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / dfrA12 / aadA2 - 1..83405 83405


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T43923 WP_001312851.1 NZ_AP027420:2618-2767 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T43923 NZ_AP027420:2618-2767 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 62 bp

>AT43923 NZ_AP027420:c2574-2513 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References