Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4100859..4101400 Replicon chromosome
Accession NZ_AP027417
Organism Escherichia coli strain EC361 isolate EC361

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag QUF17_RS23195 Protein ID WP_000615982.1
Coordinates 4100859..4101137 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QUF17_RS23200 Protein ID WP_000729704.1
Coordinates 4101140..4101400 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF17_RS23180 (4098441) 4098441..4099019 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
QUF17_RS23185 (4099225) 4099225..4099992 + 768 WP_000333380.1 class II glutamine amidotransferase -
QUF17_RS23190 (4099963) 4099963..4100703 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QUF17_RS23195 (4100859) 4100859..4101137 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QUF17_RS23200 (4101140) 4101140..4101400 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QUF17_RS23205 (4101586) 4101586..4102359 + 774 WP_000543899.1 C40 family peptidase -
QUF17_RS23210 (4102416) 4102416..4102772 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
QUF17_RS23215 (4102765) 4102765..4103043 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QUF17_RS23220 (4103148) 4103148..4105241 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
QUF17_RS23225 (4105225) 4105225..4106364 - 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T43895 WP_000615982.1 NZ_AP027417:c4101137-4100859 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T43895 NZ_AP027417:c4101137-4100859 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT43895 WP_000729704.1 NZ_AP027417:c4101400-4101140 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT43895 NZ_AP027417:c4101400-4101140 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References