Detailed information of TA system TA043892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3244468..3245070
• Replicon: chromosome
• Accession: NZ_AP027417
• Organism: Escherichia coli strain EC361 isolate EC361

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUF17_RS19220
• Protein ID: WP_000897305.1
• Coordinates: 3244468..3244779 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUF17_RS19225
• Protein ID: WP_000356397.1
• Coordinates: 3244780..3245070 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF17_RS19195 (3240382)	3240382..3240981	+	600	WP_001295269.1	glucose-1-phosphatase	-
QUF17_RS19200 (3240975)	3240975..3241847	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QUF17_RS19205 (3241844)	3241844..3242281	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QUF17_RS19210 (3242326)	3242326..3243267	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUF17_RS19215 (3243331)	3243331..3244239	-	909	WP_001162704.1	alpha/beta hydrolase	-
QUF17_RS19220 (3244468)	3244468..3244779	+	312	WP_000897305.1	hypothetical protein	Toxin
QUF17_RS19225 (3244780)	3244780..3245070	+	291	WP_000356397.1	NadS family protein	Antitoxin
QUF17_RS19230 (3245656)	3245656..3245874	+	219	WP_001295676.1	CopG family transcriptional regulator	-
QUF17_RS19235 (3246093)	3246093..3246335	+	243	WP_001087409.1	protein YiiF	-
QUF17_RS19240 (3246665)	3246665..3247594	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QUF17_RS19245 (3247591)	3247591..3248226	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUF17_RS19250 (3248223)	3248223..3249125	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43892 WP_000897305.1 NZ_AP027417:3244468-3244779 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43892 NZ_AP027417:3244468-3244779 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43892 WP_000356397.1 NZ_AP027417:3244780-3245070 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43892 NZ_AP027417:3244780-3245070 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7