Detailed information of TA system TA043870

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 357437..357808
• Replicon: chromosome
• Accession: NZ_AP027417
• Organism: Escherichia coli strain EC361 isolate EC361

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: QUF17_RS05030
• Protein ID: WP_001317028.1
• Coordinates: 357614..357808 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	357437..357615 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF17_RS04995 (352935)	352935..353870	-	936	WP_001153728.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
QUF17_RS05000 (353922)	353922..354278	-	357	Protein_356	tyrosine-type recombinase/integrase	-
QUF17_RS05005 (354364)	354364..355386	+	1023	WP_000255946.1	IS21-like element IS100 family transposase	-
QUF17_RS05010 (355383)	355383..356165	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
QUF17_RS05015 (356232)	356232..357116	-	885	Protein_359	DUF3596 domain-containing protein	-
QUF17_RS05020 (357118)	357118..357333	-	216	WP_000079604.1	excisionase XisR	-
- (357437)	357437..357615	+	179	NuclAT_0	-	Antitoxin
- (357437)	357437..357615	+	179	NuclAT_0	-	Antitoxin
- (357437)	357437..357615	+	179	NuclAT_0	-	Antitoxin
- (357437)	357437..357615	+	179	NuclAT_0	-	Antitoxin
QUF17_RS05025 (357412)	357412..357621	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
QUF17_RS05030 (357614)	357614..357808	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
QUF17_RS05035 (357865)	357865..358674	-	810	WP_000166319.1	recombination protein RecT	-
QUF17_RS05040 (358667)	358667..361267	-	2601	WP_000105127.1	exodeoxyribonuclease VIII	-
QUF17_RS05045 (361369)	361369..361644	-	276	WP_000632297.1	protein RacC	-
QUF17_RS05050 (361719)	361719..361889	-	171	WP_001352098.1	YdaE family protein	-
QUF17_RS05055 (361889)	361889..362110	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	346663..366404	19741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T43870 WP_001317028.1 NZ_AP027417:c357808-357614 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T43870 NZ_AP027417:c357808-357614 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT43870 NZ_AP027417:357437-357615 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin