Detailed information of TA system TA043863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 37286..37857
• Replicon: plasmid pEC361_3
• Accession: NZ_AP027414
• Organism: Escherichia coli strain EC361 isolate EC361

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: QUF17_RS00935
• Protein ID: WP_032323275.1
• Coordinates: 37576..37857 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUF17_RS00930
• Protein ID: WP_094259667.1
• Coordinates: 37286..37567 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF17_RS00905 (MUTS5_01730)	33193..33960	-	768	WP_016262379.1	hypothetical protein	-
QUF17_RS00910 (MUTS5_01740)	33957..34751	-	795	WP_286338137.1	phage tail protein	-
QUF17_RS00915 (MUTS5_01750)	34901..35440	+	540	WP_032152887.1	stationary phase growth adaptation protein	-
QUF17_RS00920 (MUTS5_01760)	35666..36871	-	1206	WP_286338138.1	phage tail protein	-
QUF17_RS00925 (MUTS5_01770)	36864..37193	-	330	WP_016262385.1	hypothetical protein	-
QUF17_RS00930 (MUTS5_01780)	37286..37567	-	282	WP_094259667.1	HigA family addiction module antitoxin	Antitoxin
QUF17_RS00935 (MUTS5_01790)	37576..37857	-	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF17_RS00940 (MUTS5_01800)	38193..38846	-	654	WP_021551806.1	hypothetical protein	-
QUF17_RS00945 (MUTS5_01810)	38978..39733	+	756	WP_249417410.1	hypothetical protein	-
QUF17_RS00950 (MUTS5_01820)	39754..40146	+	393	WP_223363307.1	hypothetical protein	-
QUF17_RS00955 (MUTS5_01830)	40143..40463	-	321	WP_094259664.1	transcriptional regulator	-
QUF17_RS00960 (MUTS5_01840)	40752..41462	-	711	Protein_44	Rha family transcriptional regulator	-
QUF17_RS00965 (MUTS5_01850)	41462..41677	-	216	WP_000201263.1	host cell division inhibitor Icd-like protein	-
QUF17_RS00970	41596..41814	-	219	WP_244584300.1	hypothetical protein	-
QUF17_RS00975 (MUTS5_01860)	41970..42137	-	168	WP_021551801.1	hypothetical protein	-
QUF17_RS00980	42171..42329	-	159	WP_155954547.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90284	90284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-80)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10606.14 Da
• Isoelectric Point: 10.2589

>T43863 WP_032323275.1 NZ_AP027414:c37857-37576 [Escherichia coli]
MIKSFRHKGLERFFKTGSTAGIQAKHAVKLRIQLTALNTAKRPDDMSAPGWELHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T43863 NZ_AP027414:c37857-37576 [Escherichia coli]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACGCTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTGACTGCTCTTAATACAGCTAAACGCCCGGACGATATGTCTGCGCCTGGATGGGAATTGC
ACCCACTAAAGGGGGCGGACCTTAAAGGTCACTGGGCAATATCGGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGAGAAGACGCAATTCTAGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10100.53 Da
• Isoelectric Point: 7.3179

>AT43863 WP_094259667.1 NZ_AP027414:c37567-37286 [Escherichia coli]
MSRMHNPAHPGAVLREYLGDMSVTDAAAALGITRTALSRILNEKTGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TARPNIRPLFSHA

Nucleotide

• Length: 282 bp

>AT43863 NZ_AP027414:c37567-37286 [Escherichia coli]
ATGAGTCGTATGCATAATCCCGCGCATCCTGGCGCGGTTCTTCGTGAATATTTGGGAGACATGTCAGTCACAGACGCCGC
TGCTGCATTAGGCATTACTCGCACGGCACTTTCCCGAATTCTGAACGAAAAAACAGGTATTTCTGCTGATATGGCACTTC
GTCTGGAGTCCGCTCTTGGAACCAGTGCCGAAATGTGGACCGGTATGCAATCCCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCGCTCGCCCAAACATCCGTCCATTATTTTCCCACGCATAA