Detailed information of TA system TA043815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 683530..684224
• Replicon: chromosome
• Accession: NZ_AP027408
• Organism: Escherichia coli strain EC507 isolate EC507

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: QUE45_RS04410
• Protein ID: WP_001263493.1
• Coordinates: 683826..684224 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: QUE45_RS04405
• Protein ID: WP_000554757.1
• Coordinates: 683530..683823 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE45_RS04385 (679153)	679153..679650	+	498	WP_000006245.1	REP-associated tyrosine transposase RayT	-
QUE45_RS04390 (679883)	679883..681595	-	1713	Protein_638	flagellar biosynthesis protein FlhA	-
QUE45_RS04395 (681567)	681567..682352	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
QUE45_RS04400 (682423)	682423..683478	+	1056	WP_001226164.1	DNA polymerase IV	-
QUE45_RS04405 (683530)	683530..683823	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
QUE45_RS04410 (683826)	683826..684224	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
QUE45_RS04415 (684234)	684234..684686	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
QUE45_RS04420 (684932)	684932..685135	+	204	Protein_644	RtcB family protein	-
QUE45_RS04425 (685134)	685134..685655	+	522	Protein_645	peptide chain release factor H	-
QUE45_RS04430 (685712)	685712..687169	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
QUE45_RS04435 (687430)	687430..687888	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (688484)	688484..688564	+	81	NuclAT_10	-	-
- (688484)	688484..688564	+	81	NuclAT_10	-	-
- (688484)	688484..688564	+	81	NuclAT_10	-	-
- (688484)	688484..688564	+	81	NuclAT_10	-	-
QUE45_RS04440 (687980)	687980..689224	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T43815 WP_001263493.1 NZ_AP027408:683826-684224 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T43815 NZ_AP027408:683826-684224 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT43815 WP_000554757.1 NZ_AP027408:683530-683823 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT43815 NZ_AP027408:683530-683823 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6