Detailed information of TA system TA043814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 677477..678018
• Replicon: chromosome
• Accession: NZ_AP027408
• Organism: Escherichia coli strain EC507 isolate EC507

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q32JN3
• Locus tag: QUE45_RS04370
• Protein ID: WP_000615980.1
• Coordinates: 677477..677755 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: QUE45_RS04375
• Protein ID: WP_000729707.1
• Coordinates: 677758..678018 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE45_RS04355 (675059)	675059..675637	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
QUE45_RS04360 (675843)	675843..676610	+	768	WP_000333380.1	class II glutamine amidotransferase	-
QUE45_RS04365 (676581)	676581..677321	-	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
QUE45_RS04370 (677477)	677477..677755	-	279	WP_000615980.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
QUE45_RS04375 (677758)	677758..678018	-	261	WP_000729707.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
QUE45_RS04380 (678204)	678204..678977	+	774	WP_000543885.1	C40 family peptidase	-
QUE45_RS04385 (679153)	679153..679650	+	498	WP_000006245.1	REP-associated tyrosine transposase RayT	-
QUE45_RS04390 (679883)	679883..681595	-	1713	Protein_638	flagellar biosynthesis protein FlhA	-
QUE45_RS04395 (681567)	681567..682352	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T43814 WP_000615980.1 NZ_AP027408:c677755-677477 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T43814 NZ_AP027408:c677755-677477 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT43814 WP_000729707.1 NZ_AP027408:c678018-677758 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKTKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT43814 NZ_AP027408:c678018-677758 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGACCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A090NH48