Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4477850..4478391 Replicon chromosome
Accession NZ_AP027404
Organism Escherichia coli strain EC416 isolate EC416

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag QUE37_RS23000 Protein ID WP_000615983.1
Coordinates 4478113..4478391 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag QUE37_RS22995 Protein ID WP_000729703.1
Coordinates 4477850..4478110 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE37_RS22980 (4473901) 4473901..4475994 + 2094 WP_001140826.1 flagellar biosynthesis protein FlhA -
QUE37_RS22985 (4476218) 4476218..4476715 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
QUE37_RS22990 (4476891) 4476891..4477640 - 750 WP_000056849.1 C40 family peptidase -
QUE37_RS22995 (4477850) 4477850..4478110 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QUE37_RS23000 (4478113) 4478113..4478391 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QUE37_RS23005 (4478547) 4478547..4479287 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QUE37_RS23010 (4479258) 4479258..4480025 - 768 WP_000333380.1 class II glutamine amidotransferase -
QUE37_RS23015 (4480231) 4480231..4480809 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / tssM / tssA / hcp1/tssD1 4476891..4500428 23537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T43804 WP_000615983.1 NZ_AP027404:4478113-4478391 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T43804 NZ_AP027404:4478113-4478391 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT43804 WP_000729703.1 NZ_AP027404:4477850-4478110 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT43804 NZ_AP027404:4477850-4478110 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References