Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2528774..2529276 Replicon chromosome
Accession NZ_AP027404
Organism Escherichia coli strain EC416 isolate EC416

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag QUE37_RS13125 Protein ID WP_000767829.1
Coordinates 2529022..2529276 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag QUE37_RS13120 Protein ID WP_001259255.1
Coordinates 2528774..2529025 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE37_RS13095 (2523952) 2523952..2525019 - 1068 WP_000080081.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
QUE37_RS13100 (2525019) 2525019..2526089 - 1071 WP_001710437.1 histidinol-phosphate transaminase -
QUE37_RS13105 (2526086) 2526086..2527390 - 1305 WP_004991367.1 histidinol dehydrogenase -
QUE37_RS13110 (2527396) 2527396..2528295 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
QUE37_RS13115 (2528441) 2528441..2528491 - 51 WP_001364200.1 his operon leader peptide -
QUE37_RS13120 (2528774) 2528774..2529025 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
QUE37_RS13125 (2529022) 2529022..2529276 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
QUE37_RS13130 (2529359) 2529359..2530183 + 825 WP_000754758.1 SDR family oxidoreductase -
QUE37_RS13135 (2530229) 2530229..2531158 + 930 WP_089563853.1 LysR substrate-binding domain-containing protein -
QUE37_RS13140 (2531373) 2531373..2531435 + 63 WP_010723108.1 membrane protein YoeI -
QUE37_RS13145 (2531425) 2531425..2532783 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
QUE37_RS13150 (2532962) 2532962..2534020 + 1059 Protein_2371 thiosulfate utilization transporter TsuA/YeeE -
QUE37_RS13155 (2534034) 2534034..2534261 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T43792 WP_000767829.1 NZ_AP027404:2529022-2529276 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T43792 NZ_AP027404:2529022-2529276 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT43792 WP_001259255.1 NZ_AP027404:2528774-2529025 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT43792 NZ_AP027404:2528774-2529025 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References