Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 53963..54498 Replicon plasmid L6E_p2
Accession NZ_AP027301
Organism Enterococcus hirae strain L6E

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag QUD95_RS14325 Protein ID WP_002314366.1
Coordinates 54226..54498 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag QUD95_RS14320 Protein ID WP_002297422.1
Coordinates 53963..54226 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD95_RS14300 (L6E_28150) 49142..50551 - 1410 WP_286335906.1 ATP-binding protein -
QUD95_RS14305 (L6E_28160) 50548..51231 - 684 WP_002328830.1 response regulator transcription factor -
QUD95_RS14310 (L6E_28180) 51636..52280 + 645 WP_002289351.1 IS6 family transposase -
QUD95_RS14315 (L6E_28190) 52931..53857 + 927 WP_002328829.1 hypothetical protein -
QUD95_RS14320 (L6E_28200) 53963..54226 + 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
QUD95_RS14325 (L6E_28210) 54226..54498 + 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
QUD95_RS14330 (L6E_28220) 54575..54904 + 330 WP_002292678.1 AbrB family transcriptional regulator -
QUD95_RS14335 (L6E_28230) 54894..55181 + 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
QUD95_RS14340 (L6E_28240) 55464..56321 + 858 WP_002292680.1 ParA family protein -
QUD95_RS14345 (L6E_28250) 56322..56870 + 549 WP_002292681.1 hypothetical protein -
QUD95_RS14350 57646..57998 + 353 Protein_63 S8 family serine peptidase -
QUD95_RS14355 (L6E_28270) 58035..58715 + 681 WP_002328842.1 IS6-like element ISS1N family transposase -
QUD95_RS14360 (L6E_28280) 58791..59276 + 486 WP_017547320.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia - 1..122510 122510
- flank IS/Tn - - 51636..52280 644


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T43727 WP_002314366.1 NZ_AP027301:54226-54498 [Enterococcus hirae]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T43727 NZ_AP027301:54226-54498 [Enterococcus hirae]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT43727 WP_002297422.1 NZ_AP027301:53963-54226 [Enterococcus hirae]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT43727 NZ_AP027301:53963-54226 [Enterococcus hirae]
ATGGCAAAAACTGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References