Detailed information of TA system TA043665

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1108..1525
• Replicon: plasmid pJNE151685-2
• Accession: NZ_AP027260
• Organism: Escherichia coli strain JNE151685

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QUE29_RS25270
• Protein ID: WP_223200913.1
• Coordinates: 1403..1525 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	1108..1298 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE29_RS25255	126..845	+	720	WP_001276237.1	plasmid SOS inhibition protein A	-
QUE29_RS25260	842..1156	+	315	WP_000872086.1	hypothetical protein	-
-	1105..1298	+	194	NuclAT_0	-	-
-	1105..1298	+	194	NuclAT_0	-	-
-	1105..1298	+	194	NuclAT_0	-	-
-	1105..1298	+	194	NuclAT_0	-	-
-	1108..1298	-	191	-	-	Antitoxin
QUE29_RS25265	1306..1458	+	153	Protein_3	DUF5431 family protein	-
QUE29_RS25270	1403..1525	+	123	WP_223200913.1	Hok/Gef family protein	Toxin
QUE29_RS25275	1979..2152	-	174	Protein_5	hypothetical protein	-
QUE29_RS25280	2452..2754	+	303	WP_001272236.1	hypothetical protein	-
QUE29_RS25285	2911..3198	+	288	WP_000107543.1	hypothetical protein	-
QUE29_RS25290	3317..4138	+	822	WP_001234499.1	DUF932 domain-containing protein	-
QUE29_RS25295	4434..5036	-	603	WP_077539848.1	transglycosylase SLT domain-containing protein	-
QUE29_RS25300	5357..5740	+	384	WP_001585112.1	conjugal transfer relaxosome DNA-binding protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..60729	60729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T43665 WP_223200913.1 NZ_AP027260:1403-1525 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T43665 NZ_AP027260:1403-1525 [Escherichia coli]
ATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCGGAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 191 bp

>AT43665 NZ_AP027260:c1298-1108 [Escherichia coli]
GCCTCGTGGGTTAATGAAAATTAACTACGGGGCTTTCTGCTTTCTGCCACACAACACGGTAACAAACCACCTTCACGTCA
TGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCGCACTGACCACACTCACTTTCCCTGAAAATA
ATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Antitoxin