Detailed information of TA system TA043652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3054367..3055094
• Replicon: chromosome
• Accession: NZ_AP027258
• Organism: Escherichia coli strain JNE151685

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: QUE29_RS15165
• Protein ID: WP_000547564.1
• Coordinates: 3054783..3055094 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE29_RS15160
• Protein ID: WP_000126294.1
• Coordinates: 3054367..3054786 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE29_RS15140 (3049417)	3049417..3049944	-	528	WP_001078777.1	electron transport protein HydN	-
QUE29_RS15145 (3050093)	3050093..3051103	-	1011	WP_042099590.1	DNA-binding transcriptional regulator AscG	-
QUE29_RS15150 (3051363)	3051363..3052820	+	1458	WP_021578692.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QUE29_RS15155 (3052829)	3052829..3054253	+	1425	WP_097754125.1	6-phospho-beta-glucosidase AscB	-
QUE29_RS15160 (3054367)	3054367..3054786	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QUE29_RS15165 (3054783)	3054783..3055094	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
QUE29_RS15170 (3055268)	3055268..3056011	-	744	WP_097754117.1	hypothetical protein	-
QUE29_RS15175 (3056054)	3056054..3056521	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
QUE29_RS15180 (3056514)	3056514..3056924	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
QUE29_RS15185 (3056921)	3056921..3057688	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
QUE29_RS15190 (3057688)	3057688..3058230	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QUE29_RS15195 (3058240)	3058240..3059949	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T43652 WP_000547564.1 NZ_AP027258:c3055094-3054783 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43652 NZ_AP027258:c3055094-3054783 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43652 WP_000126294.1 NZ_AP027258:c3054786-3054367 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43652 NZ_AP027258:c3054786-3054367 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5