Detailed information of TA system TA043631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4434504..4435106
• Replicon: chromosome
• Accession: NZ_AP027256
• Organism: Escherichia coli strain JNE141411

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUD91_RS21830
• Protein ID: WP_000897305.1
• Coordinates: 4434504..4434815 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUD91_RS21835
• Protein ID: WP_000356397.1
• Coordinates: 4434816..4435106 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD91_RS21800 (4429534)	4429534..4430319	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
QUD91_RS21805 (4430418)	4430418..4431017	+	600	WP_001295269.1	glucose-1-phosphatase	-
QUD91_RS21810 (4431011)	4431011..4431883	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QUD91_RS21815 (4431880)	4431880..4432317	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QUD91_RS21820 (4432362)	4432362..4433303	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUD91_RS21825 (4433367)	4433367..4434275	-	909	WP_001162704.1	alpha/beta hydrolase	-
QUD91_RS21830 (4434504)	4434504..4434815	+	312	WP_000897305.1	hypothetical protein	Toxin
QUD91_RS21835 (4434816)	4434816..4435106	+	291	WP_000356397.1	NadS family protein	Antitoxin
QUD91_RS21840 (4435692)	4435692..4435910	+	219	WP_001295676.1	CopG family transcriptional regulator	-
QUD91_RS21845 (4436129)	4436129..4436371	+	243	WP_001086388.1	protein YiiF	-
QUD91_RS21850 (4436701)	4436701..4437630	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QUD91_RS21855 (4437627)	4437627..4438262	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUD91_RS21860 (4438259)	4438259..4439161	-	903	WP_117008480.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43631 WP_000897305.1 NZ_AP027256:4434504-4434815 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43631 NZ_AP027256:4434504-4434815 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43631 WP_000356397.1 NZ_AP027256:4434816-4435106 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43631 NZ_AP027256:4434816-4435106 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7