Detailed information of TA system TA043623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2292320..2292822
• Replicon: chromosome
• Accession: NZ_AP027256
• Organism: Escherichia coli strain JNE141411

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: D8A584
• Locus tag: QUD91_RS11410
• Protein ID: WP_000767828.1
• Coordinates: 2292320..2292574 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: QUD91_RS11415
• Protein ID: WP_286335635.1
• Coordinates: 2292571..2292822 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD91_RS11380 (2287335)	2287335..2287562	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
QUD91_RS11385 (2287576)	2287576..2288634	-	1059	WP_000492340.1	thiosulfate utilization transporter TsuA/YeeE	-
QUD91_RS11390 (2288813)	2288813..2290171	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
QUD91_RS11395 (2290161)	2290161..2290223	-	63	WP_010723108.1	membrane protein YoeI	-
QUD91_RS11400 (2290438)	2290438..2291367	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
QUD91_RS11405 (2291413)	2291413..2292237	-	825	WP_000754758.1	SDR family oxidoreductase	-
QUD91_RS11410 (2292320)	2292320..2292574	-	255	WP_000767828.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
QUD91_RS11415 (2292571)	2292571..2292822	-	252	WP_286335635.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
QUD91_RS11420 (2293105)	2293105..2293155	+	51	WP_001364200.1	his operon leader peptide	-
QUD91_RS11425 (2293301)	2293301..2294200	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
QUD91_RS11430 (2294206)	2294206..2295510	+	1305	WP_001493159.1	histidinol dehydrogenase	-
QUD91_RS11435 (2295507)	2295507..2296577	+	1071	WP_001564244.1	histidinol-phosphate transaminase	-
QUD91_RS11440 (2296577)	2296577..2297644	+	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10169.56 Da
• Isoelectric Point: 8.0353

>T43623 WP_000767828.1 NZ_AP027256:c2292574-2292320 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T43623 NZ_AP027256:c2292574-2292320 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9337.67 Da
• Isoelectric Point: 4.7954

>AT43623 WP_286335635.1 NZ_AP027256:c2292822-2292571 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEEMAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT43623 NZ_AP027256:c2292822-2292571 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGATGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A829G465