Detailed information of TA system TA043607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4347243..4347845
• Replicon: chromosome
• Accession: NZ_AP027254
• Organism: Escherichia coli strain JNE120442

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUE31_RS21550
• Protein ID: WP_000897305.1
• Coordinates: 4347243..4347554 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE31_RS21555
• Protein ID: WP_000356397.1
• Coordinates: 4347555..4347845 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE31_RS21520 (4342279)	4342279..4343016	+	738	WP_286356060.1	aldose 1-epimerase	-
QUE31_RS21525 (4343157)	4343157..4343756	+	600	WP_001314324.1	glucose-1-phosphatase	-
QUE31_RS21530 (4343750)	4343750..4344622	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QUE31_RS21535 (4344619)	4344619..4345056	+	438	WP_021560109.1	D-aminoacyl-tRNA deacylase	-
QUE31_RS21540 (4345101)	4345101..4346042	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUE31_RS21545 (4346106)	4346106..4347014	-	909	WP_001318161.1	alpha/beta hydrolase	-
QUE31_RS21550 (4347243)	4347243..4347554	+	312	WP_000897305.1	hypothetical protein	Toxin
QUE31_RS21555 (4347555)	4347555..4347845	+	291	WP_000356397.1	NadS family protein	Antitoxin
QUE31_RS21560 (4348450)	4348450..4348668	+	219	WP_001298592.1	CopG family transcriptional regulator	-
QUE31_RS21565 (4348883)	4348883..4349812	-	930	WP_052920312.1	formate dehydrogenase accessory protein FdhE	-
QUE31_RS21570 (4349809)	4349809..4350444	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUE31_RS21575 (4350441)	4350441..4351343	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43607 WP_000897305.1 NZ_AP027254:4347243-4347554 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43607 NZ_AP027254:4347243-4347554 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43607 WP_000356397.1 NZ_AP027254:4347555-4347845 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43607 NZ_AP027254:4347555-4347845 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7