Detailed information of TA system TA043574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3068108..3068710
• Replicon: chromosome
• Accession: NZ_AP027246
• Organism: Escherichia coli strain JNE120393

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUF33_RS15445
• Protein ID: WP_000897305.1
• Coordinates: 3068399..3068710 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUF33_RS15440
• Protein ID: WP_000356397.1
• Coordinates: 3068108..3068398 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF33_RS15415 (3064034)	3064034..3064936	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
QUF33_RS15420 (3064933)	3064933..3065568	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUF33_RS15425 (3065565)	3065565..3066494	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
QUF33_RS15430 (3066824)	3066824..3067066	-	243	WP_001087409.1	protein YiiF	-
QUF33_RS15435 (3067285)	3067285..3067503	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QUF33_RS15440 (3068108)	3068108..3068398	-	291	WP_000356397.1	NadS family protein	Antitoxin
QUF33_RS15445 (3068399)	3068399..3068710	-	312	WP_000897305.1	hypothetical protein	Toxin
QUF33_RS15450 (3068939)	3068939..3069847	+	909	WP_001162704.1	alpha/beta hydrolase	-
QUF33_RS15455 (3069911)	3069911..3070852	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUF33_RS15460 (3070897)	3070897..3071334	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QUF33_RS15465 (3071331)	3071331..3072203	-	873	WP_000920762.1	virulence factor BrkB family protein	-
QUF33_RS15470 (3072197)	3072197..3072796	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43574 WP_000897305.1 NZ_AP027246:c3068710-3068399 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43574 NZ_AP027246:c3068710-3068399 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43574 WP_000356397.1 NZ_AP027246:c3068398-3068108 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43574 NZ_AP027246:c3068398-3068108 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7