Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2208635..2209176 Replicon chromosome
Accession NZ_AP027246
Organism Escherichia coli strain JNE120393

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag QUF33_RS11425 Protein ID WP_000615976.1
Coordinates 2208898..2209176 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QUF33_RS11420 Protein ID WP_000729704.1
Coordinates 2208635..2208895 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF33_RS11395 (2204418) 2204418..2205203 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
QUF33_RS11400 (2205175) 2205175..2206887 + 1713 Protein_2236 flagellar biosynthesis protein FlhA -
QUF33_RS11405 (2206992) 2206992..2207270 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QUF33_RS11410 (2207263) 2207263..2207619 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
QUF33_RS11415 (2207676) 2207676..2208449 - 774 WP_000543895.1 C40 family peptidase -
QUF33_RS11420 (2208635) 2208635..2208895 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QUF33_RS11425 (2208898) 2208898..2209176 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QUF33_RS11430 (2209332) 2209332..2210072 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QUF33_RS11435 (2210043) 2210043..2210810 - 768 WP_286339052.1 class II glutamine amidotransferase -
QUF33_RS11440 (2211016) 2211016..2211594 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2207263..2218788 11525


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T43571 WP_000615976.1 NZ_AP027246:2208898-2209176 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T43571 NZ_AP027246:2208898-2209176 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT43571 WP_000729704.1 NZ_AP027246:2208635-2208895 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT43571 NZ_AP027246:2208635-2208895 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References