Detailed information of TA system TA043552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4425769..4426371
• Replicon: chromosome
• Accession: NZ_AP027217
• Organism: Escherichia coli strain JNE170426

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUE10_RS21615
• Protein ID: WP_000897305.1
• Coordinates: 4425769..4426080 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE10_RS21620
• Protein ID: WP_000356397.1
• Coordinates: 4426081..4426371 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE10_RS21585 (4420799)	4420799..4421584	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
QUE10_RS21590 (4421683)	4421683..4422282	+	600	WP_001295269.1	glucose-1-phosphatase	-
QUE10_RS21595 (4422276)	4422276..4423148	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QUE10_RS21600 (4423145)	4423145..4423582	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QUE10_RS21605 (4423627)	4423627..4424568	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUE10_RS21610 (4424632)	4424632..4425540	-	909	WP_001162704.1	alpha/beta hydrolase	-
QUE10_RS21615 (4425769)	4425769..4426080	+	312	WP_000897305.1	hypothetical protein	Toxin
QUE10_RS21620 (4426081)	4426081..4426371	+	291	WP_000356397.1	NadS family protein	Antitoxin
QUE10_RS21625 (4426976)	4426976..4427194	+	219	WP_001315930.1	CopG family transcriptional regulator	-
QUE10_RS21630 (4427414)	4427414..4427656	+	243	WP_001087409.1	protein YiiF	-
QUE10_RS21635 (4427986)	4427986..4428915	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QUE10_RS21640 (4428912)	4428912..4429547	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUE10_RS21645 (4429544)	4429544..4430446	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43552 WP_000897305.1 NZ_AP027217:4425769-4426080 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43552 NZ_AP027217:4425769-4426080 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43552 WP_000356397.1 NZ_AP027217:4426081-4426371 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43552 NZ_AP027217:4426081-4426371 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7