Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 279948..280489 Replicon chromosome
Accession NZ_AP027217
Organism Escherichia coli strain JNE170426

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag QUE10_RS01275 Protein ID WP_000615976.1
Coordinates 279948..280226 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QUE10_RS01280 Protein ID WP_000729704.1
Coordinates 280229..280489 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE10_RS01260 (277530) 277530..278108 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
QUE10_RS01265 (278314) 278314..279081 + 768 WP_000333380.1 class II glutamine amidotransferase -
QUE10_RS01270 (279052) 279052..279792 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QUE10_RS01275 (279948) 279948..280226 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QUE10_RS01280 (280229) 280229..280489 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QUE10_RS01285 (280675) 280675..281448 + 774 WP_000543897.1 C40 family peptidase -
QUE10_RS01290 (281505) 281505..281861 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
QUE10_RS01295 (281854) 281854..282132 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QUE10_RS01300 (282237) 282237..283949 - 1713 Protein_252 flagellar biosynthesis protein FlhA -
QUE10_RS01305 (283921) 283921..284706 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T43531 WP_000615976.1 NZ_AP027217:c280226-279948 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T43531 NZ_AP027217:c280226-279948 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT43531 WP_000729704.1 NZ_AP027217:c280489-280229 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT43531 NZ_AP027217:c280489-280229 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References