Detailed information of TA system TA043526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4440165..4440767
• Replicon: chromosome
• Accession: NZ_AP027212
• Organism: Escherichia coli strain JNE131328

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUE42_RS21915
• Protein ID: WP_000897305.1
• Coordinates: 4440165..4440476 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE42_RS21920
• Protein ID: WP_000356397.1
• Coordinates: 4440477..4440767 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE42_RS21890 (4436079)	4436079..4436678	+	600	WP_001295269.1	glucose-1-phosphatase	-
QUE42_RS21895 (4436672)	4436672..4437544	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QUE42_RS21900 (4437541)	4437541..4437978	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QUE42_RS21905 (4438023)	4438023..4438964	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUE42_RS21910 (4439028)	4439028..4439936	-	909	WP_001162704.1	alpha/beta hydrolase	-
QUE42_RS21915 (4440165)	4440165..4440476	+	312	WP_000897305.1	hypothetical protein	Toxin
QUE42_RS21920 (4440477)	4440477..4440767	+	291	WP_000356397.1	NadS family protein	Antitoxin
QUE42_RS21925 (4441372)	4441372..4441590	+	219	WP_001295676.1	CopG family transcriptional regulator	-
QUE42_RS21930 (4441809)	4441809..4442051	+	243	WP_001087409.1	protein YiiF	-
QUE42_RS21935 (4442381)	4442381..4443310	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
QUE42_RS21940 (4443307)	4443307..4443942	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUE42_RS21945 (4443939)	4443939..4444841	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43526 WP_000897305.1 NZ_AP027212:4440165-4440476 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43526 NZ_AP027212:4440165-4440476 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43526 WP_000356397.1 NZ_AP027212:4440477-4440767 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43526 NZ_AP027212:4440477-4440767 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7