Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 281158..281699 Replicon chromosome
Accession NZ_AP027212
Organism Escherichia coli strain JNE131328

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag QUE42_RS01255 Protein ID WP_000615976.1
Coordinates 281158..281436 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QUE42_RS01260 Protein ID WP_000729704.1
Coordinates 281439..281699 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE42_RS01240 (278740) 278740..279318 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
QUE42_RS01245 (279524) 279524..280291 + 768 WP_000333380.1 class II glutamine amidotransferase -
QUE42_RS01250 (280262) 280262..281002 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QUE42_RS01255 (281158) 281158..281436 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QUE42_RS01260 (281439) 281439..281699 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QUE42_RS01265 (281885) 281885..282658 + 774 WP_000543895.1 C40 family peptidase -
QUE42_RS01270 (282715) 282715..283071 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
QUE42_RS01275 (283064) 283064..283342 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QUE42_RS01280 (283447) 283447..285159 - 1713 Protein_248 flagellar biosynthesis protein FlhA -
QUE42_RS01285 (285131) 285131..285916 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 272209..283342 11133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T43505 WP_000615976.1 NZ_AP027212:c281436-281158 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T43505 NZ_AP027212:c281436-281158 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT43505 WP_000729704.1 NZ_AP027212:c281699-281439 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT43505 NZ_AP027212:c281699-281439 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References