Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4642131..4642672 Replicon chromosome
Accession NZ_AP027206
Organism Escherichia coli strain JNE082640

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag QUE04_RS22710 Protein ID WP_000615976.1
Coordinates 4642394..4642672 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QUE04_RS22705 Protein ID WP_000729704.1
Coordinates 4642131..4642391 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE04_RS22680 (4637914) 4637914..4638699 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
QUE04_RS22685 (4638671) 4638671..4640383 + 1713 Protein_4419 flagellar biosynthesis protein FlhA -
QUE04_RS22690 (4640488) 4640488..4640766 + 279 WP_047081734.1 type II toxin-antitoxin system HicA family toxin -
QUE04_RS22695 (4640759) 4640759..4641115 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
QUE04_RS22700 (4641172) 4641172..4641945 - 774 WP_000543895.1 C40 family peptidase -
QUE04_RS22705 (4642131) 4642131..4642391 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QUE04_RS22710 (4642394) 4642394..4642672 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QUE04_RS22715 (4642828) 4642828..4643568 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QUE04_RS22720 (4643539) 4643539..4644306 - 768 WP_000333380.1 class II glutamine amidotransferase -
QUE04_RS22725 (4644512) 4644512..4645090 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T43503 WP_000615976.1 NZ_AP027206:4642394-4642672 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T43503 NZ_AP027206:4642394-4642672 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT43503 WP_000729704.1 NZ_AP027206:4642131-4642391 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT43503 NZ_AP027206:4642131-4642391 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References