Detailed information of TA system TA043483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 541852..542454
• Replicon: chromosome
• Accession: NZ_AP027206
• Organism: Escherichia coli strain JNE082640

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUE04_RS02545
• Protein ID: WP_000897305.1
• Coordinates: 542143..542454 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE04_RS02540
• Protein ID: WP_000356397.1
• Coordinates: 541852..542142 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE04_RS02515 (537778)	537778..538680	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
QUE04_RS02520 (538677)	538677..539312	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUE04_RS02525 (539309)	539309..540238	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QUE04_RS02530 (540568)	540568..540810	-	243	WP_001086388.1	protein YiiF	-
QUE04_RS02535 (541029)	541029..541247	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QUE04_RS02540 (541852)	541852..542142	-	291	WP_000356397.1	NadS family protein	Antitoxin
QUE04_RS02545 (542143)	542143..542454	-	312	WP_000897305.1	hypothetical protein	Toxin
QUE04_RS02550 (542683)	542683..543591	+	909	WP_001162704.1	alpha/beta hydrolase	-
QUE04_RS02555 (543655)	543655..544596	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QUE04_RS02560 (544641)	544641..545078	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QUE04_RS02565 (545075)	545075..545947	-	873	WP_000920762.1	virulence factor BrkB family protein	-
QUE04_RS02570 (545941)	545941..546540	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43483 WP_000897305.1 NZ_AP027206:c542454-542143 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43483 NZ_AP027206:c542454-542143 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43483 WP_000356397.1 NZ_AP027206:c542142-541852 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43483 NZ_AP027206:c542142-541852 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7