Detailed information of TA system TA043469

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 5647114..5647526
• Replicon: chromosome
• Accession: NZ_AP027197
• Organism: Escherichia coli strain 02E060

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A0F3WCK2
• Locus tag: QMG97_RS28885
• Protein ID: WP_000132621.1
• Coordinates: 5647114..5647455 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	5647450..5647526 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG97_RS28865 (5643124)	5643124..5644536	-	1413	WP_000199352.1	PLP-dependent aminotransferase family protein	-
QMG97_RS28870 (5644713)	5644713..5644877	+	165	WP_000394276.1	DUF1127 domain-containing protein	-
QMG97_RS28875 (5644974)	5644974..5645856	+	883	Protein_5641	DUF262 domain-containing protein	-
QMG97_RS28880 (5645904)	5645904..5647067	+	1164	WP_071531326.1	DUF1524 domain-containing protein	-
QMG97_RS28885 (5647114)	5647114..5647455	-	342	WP_000132621.1	endoribonuclease SymE	Toxin
- (5647450)	5647450..5647526	+	77	NuclAT_28	-	Antitoxin
- (5647450)	5647450..5647526	+	77	NuclAT_28	-	Antitoxin
- (5647450)	5647450..5647526	+	77	NuclAT_28	-	Antitoxin
- (5647450)	5647450..5647526	+	77	NuclAT_28	-	Antitoxin
- (5647450)	5647450..5647526	+	77	NuclAT_29	-	Antitoxin
- (5647450)	5647450..5647526	+	77	NuclAT_29	-	Antitoxin
- (5647450)	5647450..5647526	+	77	NuclAT_29	-	Antitoxin
- (5647450)	5647450..5647526	+	77	NuclAT_29	-	Antitoxin
QMG97_RS28890 (5647676)	5647676..5649445	-	1770	WP_000110079.1	restriction endonuclease subunit S	-
QMG97_RS28895 (5649445)	5649445..5650914	-	1470	WP_001341289.1	N-6 DNA methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12342.12 Da
• Isoelectric Point: 7.8218

>T43469 WP_000132621.1 NZ_AP027197:c5647455-5647114 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESE
LMQSLRQVCKLSARKQKQVQDFIGVITGKQKVA

Nucleotide

• Length: 342 bp

>T43469 NZ_AP027197:c5647455-5647114 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCACCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTAATTAC
CGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT43469 NZ_AP027197:5647450-5647526 [Escherichia coli]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0F3WCK2

Antitoxin